INNEMATO Operational Group: Innemato validates a protocol using ozone and microorganisms to control nematodes in tomatoes and strawberries. It replaces chemicals, restores soil health, and sustainably improves productivity.

A replicable and transferable protocol for initial soil diagnosis and nematode presence analysis. This includes microbiological evaluation through the enumeration of aerobic mesophiles, fungi, actinomycetes, and yeasts, as well as the quantification of total nematodes. These analyses allow for establishing a baseline of the soil's biological status, identifying potential phytosanitary risks, and guiding decision-making for nematode management and control in the agricultural system.

Ozone application system installed and operational, with an ozone generator integrated into the irrigation system. This includes the acquisition and installation of the ozone generator, initial testing to evaluate its oxidizing power in water, and selection of the optimal oxidizing power level for application. This system ensures the effectiveness of ozone treatment, guaranteeing controlled and safe disinfection in agricultural irrigation.

Selection and validation of beneficial microbial consortia, with strains exhibiting nematicidal and biostimulant effects, identified and compatible with each other. This result includes the search, identification, and selection of microorganisms with the potential to improve soil health and control plant-parasitic nematodes, ensuring the efficacy and compatibility of the microbial consortia for their application in agricultural systems.

Encapsulated formulation of functional microorganisms, with pilot production and validation under simulated conditions. This includes the preparation of the selected microorganisms for encapsulation, the encapsulation process itself, and the study of the microorganisms' survival conditions. This ensures the viability, stability, and functionality of the microbial consortia for their subsequent practical application in agricultural soil.

Practical protocol for preparing trial plots. This includes the initial treatment of the trial land, the design and development of distinct treatments in triplicate, and the monitoring of treatments during cultivation. Implementing this protocol ensures the standardization and reproducibility of the trials, facilitating the comparative evaluation of nematode control strategies and the effectiveness of microbial consortia under real agricultural conditions.

Evaluation of the protocol's impact on nematodes, soil, and yield, by comparison with conventional treatments. This includes the execution of analyses in the trial plots and the subsequent interpretation and discussion of the results obtained. The evaluation allows us to determine the protocol's effectiveness in reducing nematodes, its effect on soil biological quality, and its impact on crop yield, providing practical evidence for improving management strategies under real-world agricultural conditions.

Estimation of net CO₂ sequestration through quantification of soil organic carbon after application of the protocol. This result includes the design of the carbon analysis protocol, the assessment of organic matter and total carbon, the estimation of net CO₂ sequestration, and an environmental comparison integrating the results. It allows for the evaluation of the protocol's effect on carbon capture, providing practical evidence of its contribution to sustainability and its potential to mitigate emissions in agricultural systems.

The final validated technical protocol is presented in a document that includes practical guides and agronomic, environmental, and economic analyses for its replication. This outcome encompasses the detailed description of the final protocol and its validation, offering a practical and comprehensive tool that facilitates the application of the protocol in different agricultural contexts, ensures the consistency of the results, and allows for its effective adoption by technicians and farmers.

On a practical level, a work system has been defined that specifies what to communicate, who to target, and through which channels, ensuring purposeful and measurable actions. This translates into tools such as informational materials, digital content, and channels tailored to the target audience. Furthermore, resources have been developed to facilitate compliance with advertising obligations, integrating them into daily operations without creating additional burdens. Consistent application of the visual identity is also guaranteed, including mandatory elements and a homogeneous image that reinforces the project's recognition and credibility. Likewise, regulatory compliance, especially regarding data protection, is ensured by incorporating it into processes and tools. Overall, this outcome professionalizes communication with concrete, effective instruments geared toward genuine dissemination.

Scientific and technical knowledge will be generated and disseminated regarding the development of an innovative, comprehensive, and sustainable protocol for the control of plant-parasitic nematodes in agricultural soils, especially in tomato and strawberry crops. Information about the project, its objectives, expected results, and the funding provided by the Ministry will also be shared with the public.

This outcome will enable effective knowledge transfer, with the following sub-outcomes: Facilitating the adoption of the innovations and practical solutions developed in the project by livestock farmers and field technicians. Demonstrating the applicability and benefits (economic, environmental, and animal welfare) of the results under real-world farming conditions. Communicating the methods of implementation and access to the results (Farming Plan).