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H2020 RECOVER Project: Development of innovative biotic symbiosis for the biodegradation and synthesis of plastics to solve end-of-life challenges in the agricultural and food industries

  • Type Project
  • Status Filled
  • Execution 2020 -2024
  • Assigned Budget 5.767.400,72 €
  • Scope Europeo
  • Autonomous community Andalucía; Comunitat Valenciana; Madrid, Comunidad de
  • Main source of financing Horizon 2020
  • Project website https://recover-bbi.eu/
Description

The EU's transition to a more sustainable and circular economy depends on efforts to minimize waste generation. A more circular plastics economy plays a key role in this transition. The EU-funded RECOVER project aims to address the accumulation of non-recyclable agricultural and packaging plastics, as well as microplastic pollution. It will apply biotechnological solutions, including a combination of microorganisms, novel enzymes, earthworms, and insects, to degrade plastics and transform them into value-added products. Among its ambitious objectives, RECOVER will develop processes for the bioremediation of plastic pollution in soils and compost and the biotransformation of conventional plastics into biofertilizers and biodegradable plastics for agricultural and food packaging applications. In doing so, it will contribute to closing the loop in both productive sectors while addressing current environmental challenges.

The RECOVER project has significantly advanced the understanding and management of agri-food plastic waste, despite encountering challenges in achieving complete biodegradation. Extensive efforts to degrade low-density polyethylene and other plastics revealed that their recalcitrant nature posed substantial obstacles, resulting in degradation rates insufficient for economic viability and sustainability. Although the biological and mechanical pretreatment methods developed did not completely overcome these challenges, the project made notable progress in creating innovative byproducts from waste streams. One of the project's key successes was the development of practical byproducts, such as biofertilizers and chitin-based materials, which have valuable applications in agriculture and food packaging. These advances highlight the potential for beneficial reuse of waste materials, even as complete biodegradation of plastics remains elusive. The ability to produce these byproducts represents a significant step toward more sustainable waste management practices. The project has also made progress in technology development and commercialization.

Innovations such as hyperspectral imaging systems for waste tracking and predictive models for optimizing biodegradation processes have been achieved. These advances, along with a business plan for commercializing the technologies, suggest potential for future commercial success and continued progress in effective waste management solutions. The RECOVER project has made notable progress in advancing the European bioeconomy by developing innovative technologies to degrade non-recyclable plastics and produce new bio-based products. It has demonstrated the feasibility of achieving plastic circularity through the integration of innovative biotechnology tools, resulting in valuable bioproducts for agricultural and food packaging applications.

These advances promise substantial societal benefits. The project has created 21 direct and 12 indirect jobs in various fields. From an environmental perspective, RECOVER has contributed to reducing greenhouse gas emissions through the production of biofertilizers from waste, reduced land use with chitin-based biopolymers, and improved soil health. Its efforts also support biodiversity and sustainable agricultural practices. Furthermore, the project has addressed political challenges by advocating for clearer definitions and requirements for insect excrement in fertilizers under EU regulations, although further clarification is needed for full market integration.

Description of activities

The RECOVER project addresses the challenges of agri-food plastic waste through innovative biotechnological solutions. Efforts were dedicated to identifying the types of agri-food waste plastics and developing logistical approaches for their management. Four target polymers: polystyrene (PS), low-density polyethylene (LDPE), linear low-density polyethylene (LLDPE), and polyethylene terephthalate (PET), were selected for degradation and conversion. An efficient hyperspectral imaging system was established for the sorting of these plastics. Candidate organisms and enzymes for biodegradation were identified, leading to the discovery of four microbial consortia capable of utilizing various plastics, particularly LLDPE/LDPE, and the development of two novel enzymes with significant activity on LLDPE.

Additionally, the use of earthworms for soil bioremediation and vermicomposting, and insects for plastic ingestion, was explored, initiating the scaling-up of these solutions. The formulations of the selected biotechnological tools were improved, and protocols for their combined application at a pilot scale were defined. These tools were tested both ex situ (composting/vermicomposting and insect culture) and in situ (soil bioremediation) for the degradation and conversion of agri-food plastic waste. Subsequent value-added products, such as biofertilizers and chitin-based materials, were developed. These efforts were supported by techno-economic analyses, risk and social assessments, and the proposal of a preliminary logistics plan to implement the RECOVER value chain in a rural area.

Despite extensive testing, complete degradation of agri-food waste plastics, especially LDPE, was not achieved. Adjustments to biodegradation protocols did not yield effective results in complete degradation, although it was confirmed that the plastics could be chemically modified and broken down into smaller pieces by worms and insects assisted by microorganisms. A predictive model was developed to simulate biodegradation under various conditions. Byproducts of the biodegradation processes were used to create biofertilizers and chitin-based products, such as mulch coatings and food packaging films. These products proved effective in extending shelf life, controlling plant pathogens, and promoting beneficial microorganisms in crops. To enhance the visibility and impact of the RECOVER project, comprehensive communication and outreach measures were implemented. This included a communication campaign utilizing the project website, social media, press releases, and event participation. The project's results were shared through 23 scientific publications and several workshops and conferences.

In addition, the project sought external support from Horizon Results Booster for two services: improving exploitation strategies for key results, including hyperspectral imagery, predictive biodegradation models, and biofertilizers, and developing a business plan to advance the hyperspectral imaging system toward market readiness.

Contextual description

The RECOVER project addresses the critical problem of plastic pollution, particularly in the agricultural and food packaging sectors, which contribute significantly to the global plastic waste problem.

Currently, only a small portion of this waste is recycled, while the majority is incinerated, landfilled, or released into the environment. The heterogeneity and contamination of food packaging waste make recycling difficult with existing technologies, requiring innovative solutions to close the loop on plastic materials. To address this challenge, RECOVER develops biotechnological tools that use enzymes and organisms, such as microorganisms, earthworms, and insects, to degrade plastic waste and convert it into biofertilizers and bioplastics. These tools are tested under real-world conditions to ensure they can be effectively applied on farms and in municipalities, promoting a circular economy. By focusing on the degradation and transformation of non-recyclable plastics, the project seeks to reduce environmental pollution and support sustainable agricultural and packaging practices.

The RECOVER project demonstrated significant advances in the treatment of agri-food plastic waste through biotechnological solutions. By identifying organisms and enzymes for biodegradation and developing sorting systems, the project lays the foundation for innovative plastic waste management. While the complete degradation of certain plastics remains a challenge, the project successfully demonstrated that they can be modified and broken down into smaller pieces and transformed into valuable byproducts.

These byproducts include biofertilizers and chitin-based materials, such as flower pots, food packaging, and mulch films. Despite some limitations, the RECOVER project provides a path toward more sustainable and effective plastic waste management practices.

Objectives

RECOVER is a disruptive proposal by RIA, based on the real need to address the contamination of agricultural fields with non-biodegradable agricultural plastics, as well as to improve municipal waste management by drastically reducing the fraction of packaging destined for unsustainable waste management systems. We aim to achieve our ambitious goals by applying biotechnological solutions that include symbiotic bioprocesses between previously described high-performance microorganisms, using microbiological systems transmitted by various insect species.

In fact, one of the main innovations of our proposal is the combination of endogenous and exogenous microorganisms and their inoculation into insects. This will allow for higher levels of biodegradation and, simultaneously, the direct conversion of agri-food plastic waste into insect byproducts such as chitin, which can be converted into chitosan (highly valuable as a raw material for bioplastics and other interesting industrial applications thanks to its antimicrobial activity). This material will be used in active packaging, improved mulch films, and biofertilizers. RECOVER will contribute to providing new biotechnological solutions by using microorganisms, enzymes, and insects to degrade conventional plastic packaging waste and agricultural films, in addition to generating new raw materials for bioindustries.

The process will be optimized and carried out ex situ in composting reactors (the preferred route for non-recyclable plastics from municipal solid waste or easily collected agricultural films) or in situ in the case of bioremediation of soil contamination, for example, using mulch films, which will contribute to the long-term elimination of non-biodegradable polymers from the environment. Furthermore, RECOVER can address microplastic contamination at both the industrial and soil composting levels.

Results

The agriculture and food packaging industries consume huge amounts of plastic. Finding ways to reuse this material and reduce waste remains a major challenge. For example, around half of the used food packaging that ends up in municipal waste cannot be recycled because it contains food remains or is a mix of different plastics. This plastic waste is destined for landfills or incineration and can often end up in the soil. "Current recycling routes are insufficient to address these problems," explains RECOVER project coordinator María López of the University of Almería in Spain. "We need to find new solutions." RECOVER received funding from the Bio-Based Industries Joint Undertaking, a public-private partnership between the EU and industry. Turning plastic waste into useful products.

In response, RECOVER has pioneered new ways to turn plastic waste into useful products and eliminate existing plastic pollution. To do this, they employed biological solutions. "Our tools were microorganisms, insects, and earthworms," says López. "We combined their natural abilities to transform and eliminate plastic in three key scenarios." The first scenario focused on plastic that cannot be easily recycled. A combination of insects and microorganisms known to feed on and decompose plastic was tested.

This biological cocktail was used to break down plastic waste, creating a range of new possibilities. Manure from plastic-eating insects known as insect excrement was used to prepare fertilizers, while chitin, an antimicrobial compound extracted from insect exoskeletons, was used to produce biodegradable food packaging. "This food packaging can also extend the shelf life of food because it has antimicrobial properties," adds López. "We tested this on chicken breast in the project." Chitin and even insect parts were also used to produce agricultural films and biodegradable flower pots, respectively. "The idea is that these flower pots can be used alongside compost produced from insect excrement," explains López. Removing Contamination from Organic Waste A second scenario looked at organic waste streams, which often contain plastic. This contamination can be difficult to separate and remove, resulting in plastic contamination in compost.

To address this, the project used a particular type of earthworm, along with microorganisms, to remove this plastic. "The microorganisms can act as probiotics for the earthworms, helping them feed on the plastic and convert it into fertilizer," López notes. "This compost can be combined with the insect waste from our first scenario to produce high-value compost." The third scenario involved removing plastic contamination from the soil, this time using a different species of earthworm.

The project also looked at ways to monitor and model microplastic pollution in soil and compost. Key successes of the project included the development of a microplastic monitoring device, biodegradable pots, fertilizers, and films for food packaging. “All of these products are moving toward commercialization,” López notes. “However, removing plastic from soil proved to be more challenging.” This is something López believes the public should be aware of. “Plastics are in the environment, and there is still work to be done here,” she adds. “Our approach, however, could be part of a future solution.”

Indeed, much has been learned through the RECOVER project. "There are many biological solutions that could be used to help us achieve our recycling goals," says López. "This is just the tip of the iceberg, from a biotechnology perspective."

Coordinators
  • UNIVERSIDAD DE ALMERIA
Collaborators
  • INGREDIENT ODYSSEY SA
  • NUTRINSECT SRL
  • IDELUX ENVIRONNEMENT
  • CARTON BROS
  • UNIVERSITA DI PISA
  • ASA SPEZIALENZYME GMBH
  • ASOCIACIÓN AGRARIA DE JÓVENES AGRICULTORES
  • NATURPLAS PLASTICOS AGRICOLAS SL
  • HOCHSCHULE ALBSTADT-SIGMARINGEN
  • BRUNEL UNIVERSITY LONDON
  • OWS RESEARCH FOUNDATION PS
  • ENCO SRL
  • UNIVERSIDAD MIGUEL HERNANDEZ DE ELCHE
  • FEMTO ENGINEERING SRL
  • IRIS TECHNOLOGY SOLUTIONS, SOCIEDAD LIMITADA
  • SOCIEDAD ANONIMA AGRICULTORES DE LAVEGA DE VALENCIA