AGROALNEXT SOIL-HEALTH Project: Application of metagenomics for the characterization of soils of different uses
- Type Project
- Scope Autonómico
- Autonomous community Asturias, Principado de
- Main source of financing NextGenerationEU
- Project website Web del Proyecto
Description
Metabarcoding analysis of soil samples used for the cultivation of different legumes and application of new detection techniques for soil pathogenic microorganisms
Contextual description
The challenge was to understand the impact of organic (ecological) and conventional cultivation management on the microbiota associated with the rhizosphere of common bean (Phaseolus vulgaris L.) by applying the metabarcoding technique to rhizosphere samples from both cultivation systems and studying bacterial (16S) and fungal (ITS) diversity.
Results
Differences were detected in the bean rhizosphere depending on management practices: organic cultivation increased fungal diversity and taxa with beneficial functions, while conventional cultivation favored bacterial diversity. These results were presented at the XX International Congress on Plant-Microorganism Interaction. In collaboration with another project (INCREASE), 33 primers were developed to identify Colletotrichum, Pseudocercospora, Xanthomonas, and Fusarium, optimized using conventional PCR, representing a significant advance in the molecular diagnosis of pathogens.
Contact information
- Coordinator/entity name: Ana María Campa
- Mailing address: Not available
- Email coordinator/entity: anamaria.campanegrillo@asturias.org
- Phone: Not available
Coordinators
- SERIDA
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