IBER-ONE Operational Group: Animal health plan within the one-health framework based on autogenous vaccines to improve the sustainability of the Spanish Iberian pig

Creation and maintenance of the data-side IT infrastructure. Activity 1 will be developed: Implementation of the appropriate technological tools for creating the data lake structure and describing its operation, data integration and organization, and maintenance. A technological infrastructure will be implemented to store, organize, and centralize epidemiological, production, and health data from the farms. Its integration, operation, and maintenance mechanisms will be defined to guarantee traceability and availability. The following results are expected: A data lake infrastructure created and operational to integrate health and production data from the farms. An established system for organization, access, and maintenance that guarantees traceability and continuous availability. This activity will last 19 months.

Collection, analysis, and study of production data, antibiotic consumption data, and diagnostic results for the main digestive diseases affecting the Iberian pig farms under study. This will take place through four activities. First, production data will be collected from the farms before the application of autogenous vaccines, which will serve as a baseline for evaluating their effectiveness later. Next, a sample of animals with digestive clinical signs will be taken and analyzed in the laboratory. This will allow for the definition of the digestive health profile of each farm as a basis for the design of autogenous vaccines. Following this, the data will be analyzed to identify the production and health variables that most impact digestive pathology, which will serve as a target for health control and the effectiveness of the autogenous vaccines. Finally, a comprehensive epidemiological report will be prepared identifying the main digestive pathogens present on the farms, thus defining the pathogens that can be controlled through vaccination strategies.

Design and production of autogenous vaccines and their vaccination protocols through the study of data and the specific epidemiology of farms. This will be carried out in two activities: 1) Design of the autogenous vaccines through the study of the health outcomes obtained, and 2) Design of vaccination protocols that combine the composition and application protocol of the various autogenous vaccines, based on the epidemiological map obtained. In the first activity, specific autogenous vaccines will be designed for each farm based on the prior analysis of the pathogens. The design will include the selection of relevant strains and the appropriate formulation for their future application. In this way, each formulation will be adapted to the previously identified health and epidemiological profile. Then, in the second activity, personalized vaccination protocols will be developed for each autogenous vaccine, including its schedule, dosage, and route of administration, which will be tailored to the epidemiological map of each farm to maximize its effectiveness.

Implementation of vaccination protocols. This is carried out in two phases: 1) Manufacturing and initial application of autogenous vaccines against digestive pathogens, and 2) Manufacturing and application of autogenous vaccines for the continued implementation of the vaccination protocol. First, customized autogenous vaccines will be manufactured according to the established designs for each farm, following quality and safety standards. Then, they will be applied on the farms according to the defined protocols. This intervention marks the beginning of immunological control against the identified digestive pathogens. To confirm compliance with the vaccination protocol on the farms, new batches of autogenous vaccines will be manufactured. These will be applied in subsequent production cycles, following the established guidelines to maintain protection against digestive pathogens. This allows for the maintenance of active immunization against digestive pathogens and ensures adherence to the established vaccination protocol.

Collection of production data, antibiotic consumption data, and diagnostic results for the main digestive diseases affecting the Iberian pig farms under study after the application of the vaccination protocol. This is again carried out in two phases: 1) Comprehensive sampling of the farms under study, followed by laboratory analysis of the samples using various techniques after the application of the vaccination protocols; and 2) Collection of production and antibiotic consumption data after the application of the vaccination protocols. First, comprehensive sampling will be carried out on the farms after the application of the vaccination protocols. These samples will be analyzed in the laboratory, and laboratory data will be obtained that reflect the evolution of digestive pathogens after vaccination. This will allow for the evaluation of the effectiveness of the vaccination protocols on the farms. In addition, production and antibiotic consumption data will be collected, which will allow for the evaluation of the impact of autogenous vaccines on the health and productivity of the livestock.

Analysis and study of the data obtained after the application of the vaccination protocol. This is carried out in two activities: 1: Integrated analysis of the results obtained, in order to draw conclusions about the effectiveness of the applied health interventions, monitoring and maintenance of the data lake after the application of the autogenous vaccines; and 2: Assessment of the improvement in productivity, animal health, and antibiotic consumption after the application of the vaccination protocols. First, a comprehensive analysis of all the data obtained after the application of the autogenous vaccines will be carried out. In addition, the data lake will be monitored and kept up-to-date to guarantee the quality and availability of the information. Next, changes in productivity, animal health status, and antibiotic consumption on the farms will be evaluated. This assessment will allow us to determine the real impact of the autogenous vaccines on the sustainability of the production system. The expected results will be a significant improvement in the productivity and animal health status of the farms, as well as a reduction in antibiotic consumption.

Animal Health Plan. This plan is carried out in two phases: 1) Drafting a report summarizing the conclusions, and 2) Developing an Animal Health Plan based on the data obtained from the use of autogenous vaccines (AVs). Phase 1 involves preparing a detailed report that compiles and analyzes all the conclusions drawn from the project's results. This document will serve as the basis for future recommendations and decision-making in animal health. Using all this data, a comprehensive Animal Health Plan will be developed that incorporates the results and lessons learned from the application of autogenous vaccines in different settings. This plan will include preventative strategies, vaccination protocols, and control measures tailored to the specific needs of each production level. It will also offer clear strategies to improve disease prevention and control on pig farms.

RD1. Generation of informational content about the Operational Group and the aid received. Three activities are carried out: 1. Web dissemination: Development of a dedicated website for the Operational Group, updates throughout the project, and publication of news about the operational group on the project website and on the websites of the beneficiary members. 2. Social media publication: Preparation of press releases and publication of news about the operational group, its activities, and results on the social media platforms of the beneficiary members. 3. Dissemination of the Operational Group through other online channels: Dissemination of information about the operational group and its results to the general public on the Comparte platform of the Spanish Global Compact Network, EU-Farmbook, and AKIS. I+Porc will be primarily responsible for developing the dedicated website. In addition, together with Arahealth, it will disseminate information about the Operational Group and its results through the Comparte platform of the Spanish Global Compact Network, EU-Farmbook, and AKIS. With the support of the other beneficiaries, including ADA, press releases will be prepared for dissemination on social media.

RD2: Development of the visual identity and creation of the operational group's promotional materials. Three activities are carried out: 1. Development of the visual identity and design and printing of the group's promotional materials: Design of the logo, image, and promotional materials (PowerPoint templates for webinars, promotional materials such as brochures, posters, and roll-up banners, etc.). 2. Creation of a dossier with information about the operational group: A dossier with information about the operational group, beneficiary members, and objectives for dissemination through the various media and communication channels planned. 3. Creation of an infographic: Creation of an infographic summarizing the actions and results obtained for dissemination through the different communication channels of each participating entity, the final event, etc. i+Porc and Arahealth will be responsible for the design of the logo, image, and promotional materials. All members of the operational group will prepare a dossier with information about the operational group, beneficiary members, and objectives for dissemination. In addition, i+Porc will create the infographic summarizing the actions and results obtained for project dissemination.

RD3: Establishing contacts with various media outlets and preparing press releases and articles for publication. Three activities are carried out: 1. Establishing contacts with various media outlets: Identifying contacts in general and sector-specific media for sending press releases, exploring opportunities for participation in radio and television programs, especially at the local, regional, and national levels. 2. Writing articles for industry publications: Preparing articles for magazines (print or online) in the swine, veterinary, and pharmaceutical sectors (at least one publication per period): Suis, 3tres3, etc. 3. Writing articles for general-interest publications: Preparing articles for newspapers, general-interest magazines, and similar publications to raise awareness of the project among the general public (consumers). I+Porc and Arahealth will be responsible for contacting the various media outlets. All members will write different articles for industry publications and other general-interest magazines to promote the project.

RD4: Implementation of specific dissemination sessions for the Operational Group, presentations, and other events. Two activities will be carried out. 1. Two specific dissemination sessions for the operational group: one in-person session at the end of the first project period and another at the end of the second period. 2. Presentations at in-person sessions, trade fairs, webinars, and other events: at least two in each period on the operational group, its activities, and results. Presentations will also be given at online meetings of the i+Porc Innovation Working Group and the i+Porc Environment Working Group, as well as at other national and international sector events. Dissemination materials will be distributed. (FIGAN - March 2027 and March 2029, SALAMAQ - September, Zafra Fair - October, Fuente Pelayo - March). i+Porc and Arahealth will hold two in-person sessions for project dissemination. Finally, i+Porc, Arahealth and ADA, with the collaboration of Exopol, Piensos Costa and Grupo Solano, will be responsible for giving presentations at various face-to-face conferences, webinars and other industry events both nationally and internationally.